GraphPad Molarity Calculator

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Compound helper

Module 1 - Mass from volume and concentration

Result

292.200000 mg

mass = C x V x MW = 0.100000 x 0.050000 x 58.440000 = 0.292200 g

Module 2 - Volume from mass and concentration

Result

6.844627 mL

volume = (mass/MW)/C = (0.010000/58.440000)/0.025000 = 0.006845 L

Module 3 - Molarity from mass and volume

Result

100.000000 mM

M = (mass/MW)/V = (0.058440/58.440000)/0.010000 = 0.100000 M

Module 4 - Dilute a stock (C1V1 = C2V2)

Stock to pipette

10.000000 uL

Diluent to add

990.000000 uL

C1V1 = C2V2 => V1 = (C2 x V2)/C1 = (0.000100 x 0.001000)/0.010000 = 0.000010 L

Stock tube to pipette to final tube

Pipette 10.000000 uL stock, add 990.000000 uL diluent, final volume 1.000000 mL.

Serial dilution (expand)

Minimum steps needed: 10. Per step: transfer 500.000000 uL, add 500.000000 uL.

% solution to molarity converter (expand)

Converted value

Molarity: 171.115674 mM

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How to Use

Pick the module that matches your lab task, enter values with units, and review auto-scaled outputs plus step-by-step equations.

Formula

Mass = C x V x MW; Volume = (mass/MW)/C; M = (mass/MW)/V; Dilution uses C1V1 = C2V2; % conversions use density and molecular weight.

GraphPad molarity guide (bench workflow)

This page works like a connected lab utility: prepare stock solutions, compute unknown molarity, run single dilutions, and plan serial dilutions with practical units.

Not one calculator - a four-module workflow

Most lab prep tasks repeat the same patterns. Sometimes you know concentration and final volume and need the mass to weigh. Other times you already have mass and want target concentration, or you need to dilute an existing stock. This calculator keeps each workflow in a dedicated module so bench work is faster and less error-prone.

  • Module 1: Mass from concentration and volume.
  • Module 2: Volume from mass and concentration.
  • Module 3: Molarity from mass and volume.
  • Module 4: Dilution with C1V1 = C2V2 plus diluent volume.

Why this version is more practical

Real lab use depends on unit safety and quick interpretation. Outputs auto-select sensible units (for example ug, mg, mL, uL), and each module includes the substituted formula so students and new lab members can check the math line by line before mixing reagents.

Extended tools for daily bench tasks

  • Compound helper: common formula weights can be selected quickly.
  • Serial dilution planner: estimate steps, transfer volume, and target ladder.
  • Percent conversion: convert between % solutions and molarity with density support.
  • Share + copy: copy results or a shareable URL for lab notes.

FAQ

Can I enter mL or uL directly?
Yes. The calculator converts units internally, so you can safely enter lab-friendly units like uL and mL.
Why is molecular weight required?
Molecular weight links grams to moles. Any mass-to-molarity conversion depends on it.
Does Module 4 show diluent volume too?
Yes. Along with stock volume to pipette, it also gives the solvent/diluent volume to add.

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